How to design the 20bp target-specific sequence?
Posted: Thu 9 Mar 2017 10:13How to design the 20bp target-specific sequence?
FAQ from OriGene
The 20bp target-specific sequence should precede NGG (PAM). Please BLAST the seed region (14bp PAM-proximal) of the 20bp target sequence to make sure it’s unique along the genome to guarantee specificity.
Access to all Origenes' vectors
FAQ from OriGene
The 20bp target-specific sequence should precede NGG (PAM). Please BLAST the seed region (14bp PAM-proximal) of the 20bp target sequence to make sure it’s unique along the genome to guarantee specificity.
Access to all Origenes' vectors