ISOCRATIC SEPARATION OF ANILINES ON HALO RP-AMIDE
Posted: Fri 5 May 2017 15:37ISOCRATIC SEPARATION OF ANILINES ON HALO RP-AMIDE
HALO by AMT
Application n°21-B
TEST CONDITIONS:
Column: HALO RP-Amide, 4.6 x 50 mm
Part Number: 92814-407
Mobile Phase: 60/40-- A/B
A=0.02 M sodium phosphate buffer, pH=7.0
B= Acetonitrile
Flow Rate: 2.0 mL/min.
Pressure: 180 Bar
Temperature: 25°C
Detection: UV 254 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: ACN/ water-50/50
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
Extra column volume: ~14 μL
HALO by AMT
Application n°21-B
TEST CONDITIONS:
Column: HALO RP-Amide, 4.6 x 50 mm
Part Number: 92814-407
Mobile Phase: 60/40-- A/B
A=0.02 M sodium phosphate buffer, pH=7.0
B= Acetonitrile
Flow Rate: 2.0 mL/min.
Pressure: 180 Bar
Temperature: 25°C
Detection: UV 254 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: ACN/ water-50/50
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
Extra column volume: ~14 μL
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