ISOCRATIC SEPARATION OF NSAIDS ON HALO C18
Posted: Fri 5 May 2017 16:20ISOCRATIC SEPARATION OF NSAIDS ON HALO C18
HALO by AMT
Application n°13-NS
TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO C18
Part Number: 92814-402
Mobile Phase: 43/57 A/B
A= 0.02 M sodium phosphate buffer, pH=2.5
B=(methanol/ACN)-50/50
Flow Rate: 3.0 mL/min.
Pressure: 338 Bar
Temperature: 35°C
Detection: UV 254 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: methanol/ water-50/50
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
Extra column volume: ~14 μL
HALO by AMT
Application n°13-NS
TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO C18
Part Number: 92814-402
Mobile Phase: 43/57 A/B
A= 0.02 M sodium phosphate buffer, pH=2.5
B=(methanol/ACN)-50/50
Flow Rate: 3.0 mL/min.
Pressure: 338 Bar
Temperature: 35°C
Detection: UV 254 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: methanol/ water-50/50
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
Extra column volume: ~14 μL
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