HILIC SEPARATION OF BASIC DRUGS ON HALO PFP
Posted: Fri 12 May 2017 15:12HILIC SEPARATION OF BASIC DRUGS ON HALO PFP
HALO by AMT
Application n°22-B
TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO PFP
Part Number: 92814-409
Mobile Phase: 12/88-A/B
A= 0.01 M Ammonium formate buffer, pH=3.0
B=Acetonitrile
Flow Rate: 2.0 mL/min.
Pressure: 101 Bar
Temperature: 30 °C
Detection: UV 254 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: 75/25-water/methanol
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
Extra column volume: ~14 μL
The strong retention of these basic drugs on Halo PFP in HILIC mode allows the use of mobile phases with high organic content which enhances sensitivity when doing LC/MS.
The high efficiency of Halo Fused Core packings insure that peaks will be sharp and elute in small volumes.
HALO by AMT
Application n°22-B
TEST CONDITIONS:
Column: 4.6 x 50 mm, HALO PFP
Part Number: 92814-409
Mobile Phase: 12/88-A/B
A= 0.01 M Ammonium formate buffer, pH=3.0
B=Acetonitrile
Flow Rate: 2.0 mL/min.
Pressure: 101 Bar
Temperature: 30 °C
Detection: UV 254 nm, VWD
Injection Volume: 1.0 μL
Sample Solvent: 75/25-water/methanol
Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro
LC System: Shimadzu Prominence UFLC XR
Extra column volume: ~14 μL
The strong retention of these basic drugs on Halo PFP in HILIC mode allows the use of mobile phases with high organic content which enhances sensitivity when doing LC/MS.
The high efficiency of Halo Fused Core packings insure that peaks will be sharp and elute in small volumes.
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