Page 1 of 1

PEPTIDE PHENYL-HEXYL COMPARED TO ES-C18 AND ES-CN

PostPosted: Wed 11 Oct 2017 15:11
by Interchim
CENHANCED SELECTIVITY OF PEPTIDE PHENYL-HEXYL COMPARED TO ES-C18 AND ES-CN
HALO by AMT
Application n°159-PE

Image

TEST CONDITIONS:
Columns:
HALO 160Å Peptide ES-C18, 2.7 μm, 2.1x150mm
Part Number: 92122-702
HALO 160Å Peptide ES-CN, 2.7 μm, 2.1x150mm
Part Number: 92122-704
HALO 160Å Peptide Phenyl-Hexyl, 2.7 μm, 2.1x150mm
Mobile Phase:
A = 0.1% formic acid in water + 10mM ammonium formate
B = 50/50 n-propanol/water + 0.1% formic acid + 10mM ammonium formate (pH: 3.45)
Flow Rate: 0.4 mL/min.
Gradient: 10-60%B in 15 min.
Temperature: 60 °C
Detection: UV 220 nm, PDA
Injection Volume: 2 μL
Sample Solvent: water, 0.1% TFA
Response Time: 0.025 sec.
Data Rate: 12.5 Hz
LC System: Shimadzu Nexera
Flow Cell: 1 μL

The initial separation using ES-C18 showed inadequate resolution of peaks 5, 6. The same separation was attempted on a ES-CN column which improved resolution of peaks 5,6 but resulted in coelution of peaks 3,4. The Phenyl Hexyl column showed excellent resolution between peaks 3,4 along with peaks 5,6.

Image
Download_Application_159-PE_Interchim1710.pdf
(1001.32 KiB) Downloaded 230 times