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eGFP, FP 521XXL and FP481XXL : which one is the best?

PostPosted: Fri 11 Dec 2015 12:37
by emylil
Hi,

I would like to know if eGFP have stronger signal than FP XXL? (FP 521XXL and FP481XXL) because those have a fairly low molar absorbance, around 50,000 (emission's intensity limited)

Thanks

Re: eGFP, FP 521XXL and FP481XXL : which one is the best?

PostPosted: Fri 11 Dec 2015 13:01
by boriscell
HI!

I recommend you to try these reagents . The important timing delay between lenghts excitation and emission of the FP 521XXL or FP 481XXL allows detection of signals at many different wavelengths compare to eGFP, while maintaining a certain sensitivity.

Best regards

Re: eGFP, FP 521XXL and FP481XXL : which one is the best?

PostPosted: Fri 11 Dec 2015 15:04
by Interchim
Hello,

To complete boriscell's answer, please find below the information product for each one :

http://www.interchim.fr/ft/F/FP481X.pdf

http://www.interchim.fr/ft/F/FP521X.pdf

http://www.interchim.fr/ft/3/37151B.pdf

Best regards,

Interchim

Re: eGFP, FP 521XXL and FP481XXL : which one is the best?

PostPosted: Mon 14 Dec 2015 16:19
by emylil
Hello,

many thanks for your answers