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Dear All,

I would like to order some BH3 peptides I found in your catalog. More preciselly, Puma BH3 domain peptide and Bim BH3 domain peptide. I would like to know whether these peptides are cell permeable.
If not, whether I could use them in cellular microinjection.
Else, do they induce cyt c release when they are in contact with mitochondria?
Thanks in advance

Hello Catij,

The only cell permeable BH3 domain peptide we have is the Bak-BH3 domain peptide (Cat. No. SP1030a). We do not have direct experience with cellular microinjection, although it is very reasonable to assume, give that there are many publications on the bioactivity of these peptides, that this would work.

Some papers that reference microinjection of BH3 peptides are listed below:

1: Kominami K, Takagi C, Kurata T, Kitayama A, Nozaki M, Sawasaki T, Kuida K, Endo Y, Manabe N, Ueno N, Sakamaki K.
The initiator caspase, caspase-10beta, and the BH-3-only molecule, Bid, demonstrate evolutionary conservation in Xenopus of their pro-apoptotic activities in the extrinsic and intrinsic pathways.
Genes Cells. 2006 Jul;11(7):701-17.
PMID: 16824191 [PubMed - indexed for MEDLINE]

2: Gilley J, Ham J.
Evidence for increased complexity in the regulation of Bim expression
in sympathetic neurons: involvement of novel transcriptional and
translational mechanisms.
DNA Cell Biol. 2005 Sep;24(9):563-73.
PMID: 16153157 [PubMed - indexed for MEDLINE]

3: Shinoda S, Schindler CK, Quan-Lan J, Saugstad JA, Taki W, Simon RP, Henshall DC.
Interaction of 14-3-3 with Bid during seizure-induced neuronal death.
J Neurochem. 2003 Jul;86(2):460-9.
PMID: 12871587 [PubMed - indexed for MEDLINE]

4: Cartron PF, Juin P, Oliver L, Martin S, Meflah K, Vallette FM.
Nonredundant role of Bax and Bak in Bid-mediated apoptosis.
Mol Cell Biol. 2003 Jul;23(13):4701-12.
PMID: 12808108 [PubMed - indexed for MEDLINE]

5: Kandasamy K, Srinivasula SM, Alnemri ES, Thompson CB, Korsmeyer SJ, Bryant JL, Srivastava RK.
Involvement of proapoptotic molecules Bax and Bak in tumor necrosis
factor-related apoptosis-inducing ligand (TRAIL)-induced mitochondrial
disruption and apoptosis: differential regulation of cytochrome c and
Smac/DIABLO release.
Cancer Res. 2003 Apr 1;63(7):1712-21.
PMID: 12670926 [PubMed - indexed for MEDLINE]

6: Moreau C, Cartron PF, Hunt A, Meflah K, Green DR, Evan G, Vallette
FM, Juin P.
Minimal BH3 peptides promote cell death by antagonizing anti-apoptotic
proteins.
J Biol Chem. 2003 May 23;278(21):19426-35. Epub 2003 Mar 17.
PMID: 12642586 [PubMed - indexed for MEDLINE]

7: Juin P, Hunt A, Littlewood T, Griffiths B, Swigart LB, Korsmeyer S,
Evan G.
c-Myc functionally cooperates with Bax to induce apoptosis.
Mol Cell Biol. 2002 Sep;22(17):6158-69.
PMID: 12167710 [PubMed - indexed for MEDLINE]

8: Sugiyama T, Shimizu S, Matsuoka Y, Yoneda Y, Tsujimoto Y.
Activation of mitochondrial voltage-dependent anion channel by
apro-apoptotic BH3-only protein Bim.
Oncogene. 2002 Jul 25;21(32):4944-56.
PMID: 12118373 [PubMed - indexed for MEDLINE]

9: Shimizu S, Matsuoka Y, Shinohara Y, Yoneda Y, Tsujimoto Y.
Essential role of voltage-dependent anion channel in various forms of
apoptosis in mammalian cells.
J Cell Biol. 2001 Jan 22;152(2):237-50.
PMID: 11266442 [PubMed - indexed for MEDLINE]

10: Zamzami N, El Hamel C, Maisse C, Brenner C, Munoz-Pinedo C, Belzacq AS, Costantini P, Vieira H, Loeffler M, Molle G, Kroemer G.
Bid acts on the permeability transition pore complex to induce
apoptosis.
Oncogene. 2000 Dec 14;19(54):6342-50.
PMID: 11175349 [PubMed - indexed for MEDLINE]

11: Simonen M, Keller H, Heim J.
The BH3 domain of Bax is sufficient for interaction of Bax with itself
and with other family members and it is required for induction of
apoptosis.
Eur J Biochem. 1997 Oct 1;249(1):85-91.
PMID: 9363757 [PubMed - indexed for MEDLINE]

Best regards,
Interchim