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Bonjour,

je viens d'acquérir un kit de dosage de potentiel membranaire, le Trypan Red Plus. J'aimerai avoir votre avis concernant les sondes à associer à ce kit, laquelle me conseillez-vous?

cordialement

bonjour,

voici les sondes pour le potentiel membranaire:


http://www.interchim.fr/ft/4/46600A.pdf

J'ai trouvé cette information concernant le DisBAC2(3). Il peut etre utilise en associantion avec le Trypan Red Plus:

Trypan Red Plus™ is similar to Trypan Blue in cell permeability. It is not permeable to live cells. Compared to Trypan Blue, Trypan Red Plus is less toxic to cells, in particular, having minimal effect on cell surface receptors such as G-protein coupled receptors (GPCRs). Another advantage is that the cells can be clearly observed under microscope when Trypan Red Plus is used while Trypan Blue makes it quite difficult to see cells under microscope. Our Trypan Red Plus sodium salt is highly purified, and can be used up to 0.6 mM with minimal cell cytotoxicity.

bonne journée