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Weak fluorescence, decreased dye performance over time ?

PostPosted: Tue 9 Dec 2014 22:27
by Interchim
Why do I see weak fluorescence, decreased dye performance over time, or a film of dye remaining on the gel after post-staining?
FAQ from Biotium

The dye may have precipitated out of solution.

1/ Heat GelRed solution to 45-50°C for two minutes and vortex to dissolve.
2/ Store dye at room temperature to avoid precipitation.