Difference between R form and a C form to choose CF™ dyes?
Posted: Thu 11 Dec 2014 20:53For several CF™ dye colors, there is an R form and a C form, both having similar absorption and emission spectra. In such a case, which of the two CF™ dyes should I choose?
FAQ from Biotium
Rhodamine-based CF dyes (designated R) generally have better photostability but weaker fluorescence than their cyanine-based equivalents (designated C). Therefore, rhodamine-based near-IR CF dyes are a better choice for microscopy, while cyanine-based CF dyes are more ideal for flow cytometry, Western blotting, and other applications where photobleaching is less of a concern. Another factor to consider is the size of the dyes. Some of the cyanine-based near-IR CF dyes are much larger than the rhodamine-based equivalents. For antibody labeling, either version of the CF dyes is suitable. However, for applications where the dye size may cause a steric problem, the smaller dye may be a better choice.
FAQ from Biotium
Rhodamine-based CF dyes (designated R) generally have better photostability but weaker fluorescence than their cyanine-based equivalents (designated C). Therefore, rhodamine-based near-IR CF dyes are a better choice for microscopy, while cyanine-based CF dyes are more ideal for flow cytometry, Western blotting, and other applications where photobleaching is less of a concern. Another factor to consider is the size of the dyes. Some of the cyanine-based near-IR CF dyes are much larger than the rhodamine-based equivalents. For antibody labeling, either version of the CF dyes is suitable. However, for applications where the dye size may cause a steric problem, the smaller dye may be a better choice.