Do I have to use two aliquots of lysate ?
Posted: Tue 20 Jan 2015 16:54Do I have to use two aliquots of lysate for the dual Firefly & Renilla Luciferase Assay Kit? Promega’s Dual Luciferase® Reporter Assay allows for the consecutive readings from the same sample lysate.
FAQ from Biotium
Yes, our dual Firefly & Renilla Luciferase Assay Kit is based on a different assay setup than Promega’s Dual Luciferase® Reporter Assay Kit. You need to use two aliquots of lysate-one for the Renilla assay and one for the Firefly assay.
FAQ from Biotium
Yes, our dual Firefly & Renilla Luciferase Assay Kit is based on a different assay setup than Promega’s Dual Luciferase® Reporter Assay Kit. You need to use two aliquots of lysate-one for the Renilla assay and one for the Firefly assay.