Fix NucView™ 488 Caspase-3 S. for subsequent immunostaining?
Posted: Fri 6 Feb 2015 18:16Can I fix NucView™ 488 Caspase-3 Substrate for subsequent immunostaining?
FAQ from Biotium
Yes. We recommend a gentle fix in 2-4% paraformaldehyde for 10-15 minutes. Over-fixing can cause the signal to decrease. NucView™ 488 staining can withstand permeabilization with 0.1% Triton X-100. Methanol fixation is not recommended.
FAQ from Biotium
Yes. We recommend a gentle fix in 2-4% paraformaldehyde for 10-15 minutes. Over-fixing can cause the signal to decrease. NucView™ 488 staining can withstand permeabilization with 0.1% Triton X-100. Methanol fixation is not recommended.