Losing some apoptotic cells during processing of cells ...?
Posted: Fri 6 Feb 2015 19:00I am afraid that I’m losing some of my apoptotic cells during processing of cells from an adherent culture, how can I make sure that they are accounted for?
FAQ from Biotium
You may wish to process floating and detached cells separately – collect the washes and spin them down and then stain those cells using the protocol for suspension cells. Alternatively you may collect floating cells, detach the adherent cells with trypsin (without EDTA), pool the cells together, and use the suspension cell protocol for staining.
FAQ from Biotium
You may wish to process floating and detached cells separately – collect the washes and spin them down and then stain those cells using the protocol for suspension cells. Alternatively you may collect floating cells, detach the adherent cells with trypsin (without EDTA), pool the cells together, and use the suspension cell protocol for staining.