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The assay doesn't reflect the difference of my cells density

PostPosted: Fri 6 Feb 2015 19:14
by Interchim
I can see a difference in the density of my cells, why doesn’t the assay reflect the difference?
FAQ from Biotium

Check to make sure your cell densities are in the linear range of the assay. Too few cells may fall below the limit of detection, and too many cells may saturate the OD. Perform an initial experiment to check the best linear range for your cell type and experimental set-up. You may need to vary the cell density assay incubation time. The kit protocols provide general guidelines and may need to be optimized for your experimental system.