What is the purpose of the background wavelength?
Posted: Fri 6 Feb 2015 19:20
What is the purpose of the background wavelength? Do I have to use it?
FAQ from Biotium
It is recommended to measure the background wavelength; however, the background should be very low compared to the signal. Measuring the background confirms that there is no unexpected signal (for example, from the plate itself) that might interfere with the reading.
FAQ from Biotium
It is recommended to measure the background wavelength; however, the background should be very low compared to the signal. Measuring the background confirms that there is no unexpected signal (for example, from the plate itself) that might interfere with the reading.