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Annexin V-FITC w. cells expressing green fluorescent protein

PostPosted: Mon 16 Feb 2015 14:50
by Interchim
Can Annexin V-FITC 4830-01-01 be used with cells expressing green fluorescent protein (GFP)?
FAQ from Trevigen

TACS™ Annexin V-FITC kit cannot be used with cells expressing GFP since the fluorescence molecules have similar excitation and emission spectra.
However, the TACS™ Annexin V-Biotin Kit (cat# 4835-01-K and cat# 4835-250-K) offers flexibility in your flurophore preference. Trevigen supplies Streptavidin Conjugates compatible with the Annexin V-Biotin kit, flow cytometric-based assays, and for in situ labeling.