Troubles w. double labeling experiments the TACS™ protocol?
Posted: Mon 16 Feb 2015 15:28A paraffin embedded section was first stained using Vector Nova RED substrate kit (for peroxidase) to detect a cytoplase protein by immunohistochemistry. The section was then double labeled using TACS™ 2 TDT Kit. After completing the TACS™ protocol the red color stained by immunohistochemistry disappeared. What is the reason?
FAQ from Trevigen
For double labeling experiments the TACS™ protocol should be performed first.
The conditions to permeabilize the sample (e.g. proteinase K) should be used to optimize antigen detection (i.e. antibody dilutions etc).
The permeabilization step precedes the antibody detection step to avoid removal of antibody complexes.
FAQ from Trevigen
For double labeling experiments the TACS™ protocol should be performed first.
The conditions to permeabilize the sample (e.g. proteinase K) should be used to optimize antigen detection (i.e. antibody dilutions etc).
The permeabilization step precedes the antibody detection step to avoid removal of antibody complexes.