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Method/Kit recommended to detect apoptosis in frozen brain?

PostPosted: Mon 16 Feb 2015 15:31
by Interchim
Which method or kit is recommended to detect apoptosis in frozen brain (hippocampus) samples from a heat stressed animal? Are there any pre-treatments for thick tissue sections? Is a free floating method required?
FAQ from Trevigen

NeuroTACS™ II In Situ Apoptosis Detection Kit (cat# 4823-30-K) is recommended for convenient identification of apoptosis in brain tissue or neuronal cells.