Method/Kit recommended to detect apoptosis in frozen brain?

Which method or kit is recommended to detect apoptosis in frozen brain (hippocampus) samples from a heat stressed animal? Are there any pre-treatments for thick tissue sections? Is a free floating method required?
FAQ from Trevigen
NeuroTACS™ II In Situ Apoptosis Detection Kit (cat# 4823-30-K) is recommended for convenient identification of apoptosis in brain tissue or neuronal cells.
FAQ from Trevigen
NeuroTACS™ II In Situ Apoptosis Detection Kit (cat# 4823-30-K) is recommended for convenient identification of apoptosis in brain tissue or neuronal cells.