How much sample can I load on the TSKgel Amide-80 column 4.6 mm ID x 25 cm L?
FAQ from TOSOH
Sample loading capacity increases with decreasing polarity of the mobile phase. For example, the highest loading capacity for mannitol (200 mg) occurs in 75:25 acetonitrile:water but decreases to <100 mg in 65:35 acetonitrile:water. The maximum injection volume is 50 mL.