What assays are available to screen compounds for anti-microtubule activity?
FAQ from CYTOSKELETON
Cytoskeleton, Inc. supplies multiple kits that can be used to examine a compound’s anti-microtubule activity. We offer absorbance and fluorescence-based tubulin polymerization assay Biochem Kits to follow polymerization kinetics. The polymerization kits can be used to evaluate a compounds’s ability to induce microtubule depolymerization or impair polymerization. We also offer kits that provide a convenient means of producing either fluorescently-labeled or unlabeled microtubules. These microtubules can then be incubated with compounds and changes in the length and number of microtubules can be quantified. Additionally, the microtubule/tubulin in vivo assay kit offers a straightforward method for quantitating the in vivo ratio of tubulin polymers to monomers. This kit allows the researcher to easily analyze changes in tubulin monomers and polymers induced by various treatment conditions in cell or tissue samples.