What is the difference between TSKgel Super-ODS and ODS-140HTP columns?
FAQ from TOSOH
TSKgel Super-ODS reversed phase columns, when introduced in 1995, were the first commercially available columns packed with sub-3 micron particles. Often referred to as 2 micron packed columns, the mean particle size of the silica is 2.3 micron. The same silica lots used to prepare TSKgel Super-ODS columns are used in the manufacture of TSKgel ODS-140HTP columns.
There are two important differences between the two column types. First, although the same polymeric C18 bonding chemistry is used to produce TSKgel Super-ODS and ODS-140HTP columns, the endcapping reaction on TSKgel ODS-140HTP columns is more exhaustive than on TSKgel Super-ODS columns. In addition, TSKgel Super-ODS (as well as Super-Octyl and Super-Phenyl columns) have restrictions for flow rate as well as pressure. For instance, the 4.6 mm ID TSKgel Super-ODS columns cannot be operated above 30 MPa, nor above 4 mL/min. In contrast, the upper pressure limit for 2.1 mm ID TSKgel ODS-140HTP columns is twice as high at 60 MPa, and there is no upper limit for the flow rate, making TSKgel ODS-140HTP columns your TSKgel columns of choice for use at ultra-high pressures and flow rates.
The more exhaustive endcapping reaction that is performed on TSKgel ODS-140HTP columns means (in order of importance):
- shorter retention, higher efficiency and better peak shape for basic compounds
- slightly shorter retention of polar compounds
- improved chromatography of chelating compounds.