Cytonin offers an alternative to Proteinase K for permeabilizing cells and tissues prior to labeling using any of the TACS™ In Situ Apoptosis Detection kits. Cytonin is a protease free, saponin-based buffer designed specifically for in situ detection of apoptosis. Cytonin should be used when protease treatment must be avoided. For example, it may be used for tissues with low cellularity or little connective tissue that do not withstand protease treatment. Cytonin IHC (see below) is recommended for double labeling experiments for the detection of DNA fragmentation in conjunction with immunohistochemistry on antigens that are sensitive to proteolysis.
Cytonin IHC :
Cytonin-IHC a non-proteolytic, saponin-based permeabilization and blocking reagent designed specifically for double labeling experiments using TACS In Situ Apoptosis Detection kits, and a primary antibody of your choice. Cytonin IHC is used as a convenient time-saving antibody diluent for immunohistochemistry, allowing you to simultaneously perform your primary antibody incubation and permeabilization of your sample for in situ detection of apoptosis. In fact, use Cytonin IHC as a diluent for your secondary antibody too.