Storage and handling of tubulin
Technical Tip from CYTOSKELETON
Tubulin is a labile protein. Keep the lyophilized tubulin dry by storing in a desiccator at either 4°C or -70°C. After reconstituting tubulin as directed on the product's datasheet, experiment-sized aliquots must be snap-frozen to liquid nitrogen before storing at -70°C to preserve tubulin's activity. Tubulin should also not be frozen at a concentration below 6 mg/ml. When thawing the aliquots, thaw tubes in a room temperature water bath and immediately place on ice until used. Do not re-freeze tubulin once thawed. Throw away unused thawed tubulin aliquots.