Transposition of a Flash column from 6g to 20g:
Concerned instruments: puriFlash 215, puriFlash 430, puriFlash 450, puriFlash 4100, puriFlash 4250
Concerned module: general
Purification performed on a 6g at 20 ml/min.: rt1 elutes at 2.1 min. and rt2 elutes at 7.2 min. in isocratic or gradient mode.
We want to know the rt1 and rt2 values for a 20g Flash column at a flow rate of 10 ml/min., again in isocratic or gradient mode.
Purification using a 6g Flash column (CVo = 5 ml):
Rt1= 2.1min -> vol=40.2mL ->CV1=8
Rt2= 7.2min -> vol=144mL ->CV2=29
Purification on a 20g Flash column (CVo = 19mL):
Rt1=? I.e. 7.6 min.
Rt2=? I.e. 27.3 min.