What is the best RhoA antibody available?
FAQ from CYTOSKELETON
There are multiple options when choosing an antibody to detect small GTPases. Several factors must be considered: specificity, sensitivity, format compatibility, species cross-reactivity and consistency of signal. Cytoskeleton’s RhoA mouse monoclonal antibody scores high marks on all of these parameters. It is specific to RhoA, meaning not only will it not detect Rac or Cdc42 proteins, but it is isotype specific as well. It will not detect RhoB or RhoC. It is also very sensitive and works well for both western blotting and immunochemistry. Of course there are other companies that also make good antibodies that are comparable to ours. There have been reports of lot to lot variability with some supplier’s RhoA antibodies. Cytoskeleton’s antibodies do not have batch to batch variability due to our stringent quality control process.