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BIOCHROMATOGRAPHY > Affinity > Bio-Works Protein A
Logo_Bio_Works_Interchim_1016 Bio-Works, a Sweden company, designs, develops and manufactures a broad product range in Uppsala.
For uses within areas of Life Sciences, Food & Beverage.

WorkBeads™ Protein A
WorkBeads™ BabyBio A

WorkBeads™ Protein A & BabyBio A are designed for the purification of monoclonal and polyclonal antibodies. Made from agarose, well established and well-known in the Biotech industry.

  • Higher dynamic binding capacity for monoclonal antibodies; excellent recovery & high purity
  • Stronger coupling chemistry; high pH stability and low leakage
  • Reliable, reproducible and efficient

WorkBeads Protein A affinity chromatography media is produced from agarose using a cross-linking method that results in a highly porous and physically stable agarose matrix. Agarose matrices have successfully been used for many years in biotechnology research and in the industrial purification of proteins.

The recombinant protein A is engineered to facilitate an oriented coupling to the matrix which allows high binding capacities for target proteins. The high capacity, chemical stability and low ligand leakage make WorkBeads Protein A ideal for purification of mono- and polyclonal antibodies.

WorkBeads™ Protein A is designed to allow oriented coupling to the bead via multipoint attachment. This allows high utilization of the immobilized protein A resulting in high IgG binding conditions.
Bead has a dynamic binding capacity of typically more than 40 mg IgG/ml media under standard binding conditions (PBS, pH 7.4 and 3 minutes residence time).


Media Characteristics

Medium WorkBeads Protein A WorkBeads Protein A
Target substance Antibodies (IgG), bound via the Fc-region
Matrix Rigid, highly cross-linked agarose
Ligand Recombinant protein A expressed in E. coli using animal-free medium
Coupling chemistry Bromohydrin
Dynamic binding capacity (DBC) >40 mg human IgG/ml medium
Recommended flow rate 250 cm/h
Max flow rate conditions (see Data Sheet) 500 cm/h
Chemical stability Compatible with all standard aqueous buffers used for protein purification, and 10 mM HCl (pH2),10 mM NaOH (pH 12), 0.1M sodium citrate-HCl (pH 3), 6 M guanidine-HCl, 20% ethanol. Should not be stored at low pH for prolonged time.
Recommended working range pH Stability 3-10 short term
2-12 cleaning
Storage +2°C to +8°C in 20% ethanol

 

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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 5ML MINICOLUMN
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P/N : 45400005     Pack : 1 x 5 ml  
P/N : 45400005         Item : A2ZAR0         Pack : 1 x 5 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 1ML MINICOLUMN
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P/N : 45400001     Pack : 1 x 1 ml  
P/N : 45400001         Item : A2ZAQ0         Pack : 1 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 1ML MINICOLUMN
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P/N : 45400002     Pack : 2 x 1 ml  
P/N : 45400002         Item : A2ZAQ1         Pack : 2 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 1ML MINICOLUMN
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P/N : 45400003     Pack : 5 x 1 ml  
P/N : 45400003         Item : A2ZAQ2         Pack : 5 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 1ML MINICOLUMN
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P/N : 45400004     Pack : 10 x 1 ml  
P/N : 45400004         Item : A2ZAQ3         Pack : 10 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 5ML MINICOLUMN
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P/N : 45400008     Pack : 10 x 5 ml  
P/N : 45400008         Item : A2ZAS2         Pack : 10 x 5 ml  
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BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 5ML MINICOLUMN
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P/N : 45400006     Pack : 2 x 5 ml  
P/N : 45400006         Item : A2ZAS0         Pack : 2 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO ACT AFFINITY 5ML MINICOLUMN
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P/N : 45400007     Pack : 5 x 5 ml  
P/N : 45400007         Item : A2ZAS1         Pack : 5 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1ML MINICOLUMN
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P/N : 45605101     Pack : 1 x 1 ml  
P/N : 45605101         Item : 1M1790         Pack : 1 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1ML MINICOLUMN
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P/N : 45605104     Pack : 10 x 1 ml  
P/N : 45605104         Item : 1M1793         Pack : 10 x 1 ml  
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BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1ML MINICOLUMN
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P/N : 45605103     Pack : 5 x 1 ml  
P/N : 45605103         Item : 1M1792         Pack : 5 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 1ML MINICOLUMN
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P/N : 45605102     Pack : 2 x 1 ml  
P/N : 45605102         Item : 1M1791         Pack : 2 x 1 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5ML MINICOLUMN
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P/N : 45605108     Pack : 10 x 5 ml  
P/N : 45605108         Item : 1M1803         Pack : 10 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5ML MINICOLUMN
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P/N : 45605107     Pack : 5 x 5 ml  
P/N : 45605107         Item : 1M1802         Pack : 5 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5ML MINICOLUMN
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P/N : 45605106     Pack : 2 x 5 ml  
P/N : 45605106         Item : 1M1801         Pack : 2 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS BIO-WORKS BABYBIO A PROTEIN A AFFINITY 5ML MINICOLUMN
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P/N : 45605105     Pack : 1 x 5 ml  
P/N : 45605105         Item : 1M1800         Pack : 1 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS WORKBEADS 40 PROTEIN A 40UM
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P/N : 40605005     Pack : 01 x 1 L  
P/N : 40605005         Item : A2ZAP4         Pack : 01 x 1 L  
Cat. Price :




 
 
 
BIO-WORKS WORKBEADS 40 PROTEIN A 40UM
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P/N : 40605004     Pack : 01 x 100 ml  
P/N : 40605004         Item : A2ZAP3         Pack : 01 x 100 ml  
Cat. Price :




 
 
 
BIO-WORKS WORKBEADS 40 PROTEIN A 40UM
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P/N : 40605003     Pack : 01 x 10 ml  
P/N : 40605003         Item : A2ZAP2         Pack : 01 x 10 ml  
Cat. Price :




 
 
 
BIO-WORKS WORKBEADS 40 PROTEIN A 40UM
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P/N : 40605002     Pack : 01 x 5 ml  
P/N : 40605002         Item : A2ZAP1         Pack : 01 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS WORKBEADS 40 PROTEIN A 40UM
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P/N : 40605001     Pack : 01 x 1.5 ml  
P/N : 40605001         Item : A2ZAP0         Pack : 01 x 1.5 ml  
Cat. Price :




 
 
 
BIO-WORKS WORKBEADS 40 PROTEIN A 40U
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P/N : 40605001     Pack : 01 x 1.5 ml  
P/N : 40605001         Item : 7A2040         Pack : 01 x 1.5 ml  
Cat. Price :




 
 
 
BIO-WORKS WORKBEADS 40 PROTEIN A 40U
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P/N : 40605002     Pack : 01 x 5 ml  
P/N : 40605002         Item : 7A2041         Pack : 01 x 5 ml  
Cat. Price :




 
 
 
BIO-WORKS WORKBEADS 40 PROTEIN A 40U
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P/N : 40605003     Pack : 01 x 10 ml  
P/N : 40605003         Item : 7A2042         Pack : 01 x 10 ml  
Cat. Price :




 
 
 
BIO-WORKS WORKBEADS 40 PROTEIN A 40U
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P/N : 40605004     Pack : 01 x 100 ml  
P/N : 40605004         Item : 7A2043         Pack : 01 x 100 ml  
Cat. Price :




 
 
 
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