Bio-Works, a Sweden company, designs, develops and manufactures a broad product range in Uppsala.
For uses within areas of Life Sciences, Food & Beverage.
WorkBeads™ Protein A
WorkBeads™ BabyBio A
WorkBeads™ Protein A & BabyBio A are designed for the purification of monoclonal and polyclonal antibodies. Made from agarose, well established and well-known in the Biotech industry.
Higher dynamic binding capacity for monoclonal antibodies; excellent recovery & high purity
Stronger coupling chemistry; high pH stability and low leakage
Reliable, reproducible and efficient
WorkBeads Protein A affinity chromatography media is produced from agarose using a cross-linking method that results in a highly porous and physically stable agarose matrix. Agarose matrices have successfully been used for many years in biotechnology research and in the industrial purification of proteins.
The recombinant protein A is engineered to facilitate an oriented coupling to the matrix which allows high binding capacities for target proteins. The high capacity, chemical stability and low ligand leakage make WorkBeads Protein A ideal for purification of mono- and polyclonal antibodies.
WorkBeads™ Protein A is designed to allow oriented coupling to the bead via multipoint attachment. This allows high utilization of the immobilized protein A resulting in high IgG binding conditions.
Bead has a dynamic binding capacity of typically more than 40 mg IgG/ml media under standard binding conditions (PBS, pH 7.4 and 3 minutes residence time).
Medium WorkBeads Protein A
WorkBeads Protein A
Antibodies (IgG), bound via the Fc-region
Rigid, highly cross-linked agarose
Recombinant protein A expressed in E. coli using animal-free medium
Dynamic binding capacity (DBC)
>40 mg human IgG/ml medium
Recommended flow rate
Max flow rate conditions (see Data Sheet)
Compatible with all standard aqueous buffers used for protein purification, and 10 mM HCl (pH2),10 mM NaOH (pH 12), 0.1M sodium citrate-HCl (pH 3), 6 M guanidine-HCl, 20% ethanol. Should not be stored at low pH for prolonged time.