Anti-Mouse IgG (H+L) Goat Alkaline Phosphatase Liquid, Sterile filtered - Code E983

Directions for Use

Affinity Purified (minimum cross reactivity to bovine, chicken, guinea pig, hamster, horse, human, mouse, rabbit, rat and sheep serum proteins)

Label

Alkaline Phosphatase (Calf Intestine)

Dilution Information

This product has been assayed against 5 g of Mouse IGG by an antibody capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.

Suggested working dilution:  1:5,00 - 12,000

Concentration

1 mg/ml (by UV absorbance at 280 nm)

Buffer (pH:8.0)

0.05M Tris Hydrochloride

0.15M Sodium Chloride

1mM Magnesium Chloride

0.1mM Zinc Chloride

50% (v/v) Glycerol

Stabilizer

10 mg/ml Bovine Serum Albumin (BSA)

Preservative

0.05% (w/v) Sodium Azide

Storage

4C before opening.  DO NOT FREEZE.  Aliquot and store at 4C. Dilute only prior to immediate use.  Expiration date is 6 months from opening.

Purity

This product has been prepared from monospecific antisera by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by multiple solid phase adsorptions to remove cross reactivity. A single precipitin arc was observed against Mouse IgG, Mouse serum, anti-Alkaline Phosphatase and anti-goat serum when assayed by immunoelectrophoresis.  No reaction was observed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit, rat or sheep serum proteins.

Immunogen

Mouse IgG, Whole molecule

FOR RESEARCH USE ONLY.  NOT FOR DIAGNOSTIC OR THERAPEUTIC USE

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