Anti-Rat IgG (H+L) Goat Alkaline Phosphatase Liquid, Sterile filtered - Code E966

Directions for Use

Affinity Purified (minimum cross reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit and sheep serum proteins)

Label

Alkaline Phosphatase (Calf Intestine)

Dilution Information

This product has been assayed against 5 g of Rat IgG by an antibody capture ELISA using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.

Suggested working dilution:  1:700 - 1:7,000

Concentration

1 mg/ml (by UV absorbance at 280 nm)

Buffer (pH:8.0)

0.05M Tris Hydrochloride

0.15M Sodium Chloride

1mM Magnesium Chloride

0.1mM Zinc Chloride

50% (v/v) Glycerol

Stabilizer

10 mg/ml Bovine Serum Albumin (BSA)

Preservative

0.05% (w/v) Sodium Azide

Storage

4°C before opening.  DO NOT FREEZE.  Aliquot and store at 4°C. Dilute only prior to immediate use.  Expiration date is 6 months from opening.

Purity

This product has been prepared from monospecific antisera by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by multiple solid phase adsorptions to remove cross reactivity. A single precipitin arc was observed against Rat IgG, Rat serum, anti-Alkaline Phosphatase and anti-goat serum when assayed by immunoelectrophoresis.  No reaction was observed against bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep serum proteins.

Immunogen

Mouse IgG, Whole molecule

Note: This product is for research use only and is not intended for therapeutic or diagnostic applications.  Please contact a technical service representative for more information.

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