In situ Hybridization using HISTOCHOICE^TM MB - Code H120

Directions for Use

This fixative is designed as a substitute for formalin and can be used in your normal protocol (with the following application notes to be considered):

Fixation times:

If you are currently fixing for longer periods, you may maintain your present protocol.

HISTOCHOICE MB is a non-crosslinking fixative. Tissues will be softer and will have a more natural appearance than formalin fixed tissues. If firmer tissue is desired, use 4 parts
HISTOCHOICE MB to 1 part ethanol.

DO NOT Predigest!  If your current protocol contains a predigestion step (using Proteinase K, Trypsin, etc.), this step MUST be omitted. HISTOCHOICE MB does not crosslink tissue; therefore, no predigestion is required.

After perfecting your in situ hybridization or immunohistochemistry procedure using HISTOCHOICE MB, you may find that you can decrease your primary probe or primary antibody concentration by 1/2 - 1/10% and obtain the same or better results.

The osmolality of a 1:10 dilution of this fixative in diH₂O is approximately 340 - 360 mOsm/kg H₂O. Adjustments will need to be made with sodium chloride or sucrose if working with marine (saltwater) organisms.

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