Mouse monoclonals against Rat immunoglobulins

Mouse IgG1k - clone MARD-3

Reactivity :

Rat delta heavy chain of immunoglobulin (determined by immunodot)

Avidity :

on rat IgD: 1.1 x 1010 M-1 . Cf Avidity sheet, for more details.

Applications :

Cf Reactivity
- can be used for immunopurification of rat IgD protein (Solid phase Sepharose 4B CNBr act.)
- can be used as second antibody in immunoassays
- can be labelled with FITC, TRITC and biotin
- can be used for immunofluorescence
- can be used for capture elisa: good binding on plastics.
- can be used in western blot analysis (denaturated and non denaturated conditions)
- can be coated on nitrocellulose (dot-elispot)
- can be used on cryosections (immunohistology) as first antibody (purified or labelled with FITC)

Lyophilization :

not tested

References

(1) Bazin H., Beckers A., Urbain-Vansanten G., Pauwels R., Bruyns C., Tilkin A.F., Platteau B. & Urbain J. Transplantable IgD immunoglobulin- secreting tumours in rats. J. Immunol. 1978,121,2077-2082

Selected References of scientific publications in which MARD-3 MAb was used: Manouvriez P. & Bazin. J. Immunol. 1984,133,3274- 3281; Chavez V. et al. Int. Arch. Allergy Immunol., 1992,97:330-336; Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8; Soares M. et al. Transplantation 1993, 56: 1427-1433; Vanhove B., Bazin H. Immunology 1993, 79: 580-586; Latinne et al. Immunol. Rev. 1994, 141: 95-125; Soares et al. Transplant. Proc. 1995, 27: 282-285

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