Mouse monoclonals against Rat immunoglobulins

Mouse IgG1k - clone MARE-1

Immunogen :

IgE (IR162, IR410) (1)

Reactivity :

Rat epsilon heavy chain of immunoglobulin (C epsilon 3) (4) (determined by immunodot)

Avidity :

On rat IgE: 4 x 109 M-1 (Cf. Avidity sheet, for more details).

Applications :

Cf Reactivity
- can be used as second antibody in immunoassays (2,3,4,5,6)
- can be labelled with iodine (2,3), biotin, FITC and peroxidase
- can be used for capture elisa: good binding on plastics
- can be used in western blot analysis: binds to rat IgE in denaturated and non denaturated conditions.
- suitable for degranulation of rat mast cells (positive reaction in PCA)
- can be used in immunoaffinity chromatography for purification of Rat IgE (Solid phase Sepharose 4B CNBr act.)
- can be coated on nitrocellulose (dot-elispot)
- can be used on cryosections (immunohistology) as first antibody labelled with FITC

Lyophilization :

Yes, with a possible low denaturation.

References

(1) Bazin H., Querinjean P., Beckers A., Heremans J.F. & Dessy F.Transplantable immunoglobulin-secreting tumours in rats.IV.Sixty-three IgE-secreting immunocytoma tumours. Immunology 1974,26:713-723

(2) Manouvriez P., Ravoet A.M. & Bazin H. Fc epsilon receptors on rat B- lymphocytes: specificity and binding kinetics. Mol. Immunology 1985,22:1201-1208

(3) Manouvriez P. & Bazin H. In vivo kinetics and nature of rat IgE- bearing lymphocytes after IgE stimulation. J. Immunol. 1984,133:3274- 3281

(4) Rousseaux-Prevost R., Rousseaux J., Bazin H. Studies of the IgE binding sites to rat mast cell receptor with proteolytic fragments and with a monoclonal antibody directed against epsilon heavy chain: evidence that the combining sites are located in the C epsilon 3 domain. Molecular Immunology 1987, 24, 187-196

(5) Pluschke G. and Bordmann G. Isolation of rat immunoglobulin class switch variants of rat-mouse hybridomas by enzyme-linked immunosorbent assay and sequential sublining. Eur. J. Immunol. 1987, 17, 413-416.

(6) Manouvriez P., Lefebvre M., Genart C., Cormont F. & Bazin H. Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by Elisa with mouse monoclonal antibodies. Immunobiology 1986, 173, 307-308.

Selected References of scientific publications in which MARE-1 MAb was used: Manouvriez P.et al. Ann. Inst. Pasteur, 1985, 136C:187-193; Rousseaux-Prevost R., et al. Mol. Immunol., 1987, 24:187-196; Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8;Vanhove B., Bazin H. Immunology 1993, 79: 580-586; Yamada M., et al. Immunology 1993, 78: 298-302; Soares M., et al., Transplant. Proc. 1995, 27: 282-285.

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