Mouse monoclonals against Rat immunoglobulins

Mouse IgG1k - clone MARG1-1

Mouse IgG1k - clone MARG1-2

Mouse IgG1k - clone MARG1-1

Immunogen :

IgG1 (IR27, IR31) (1) (2)

Reactivity :

Rat gamma 1 heavy chain of immunoglobulin (determined by ELISA) (2)
The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.

Avidity :

on rat IgG1: 1 x 1011 M-1 - Cf. Avidity sheet, for more details.

Applications :

Cf Reactivity
- can be labelled with biotin, peroxidase
- can be used as second antibody in immunoassays
- can be used for capture elisa: good binding on plastics

Lyophilization :

not tested

References

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunol. 1974,4:44-48

(2) Querinjean P., Bazin H., Starace V., Beckers A., Deckers C. & Heremans J.F. L light chains in rat immunoglobulins. Immunochemistry 1973,10:653-654

Mouse IgG1k - clone MARG1-2

Immunogen :

IgG1(IR27, IR31) (1) (2)

Reactivity :

Rat gamma 1 heavy chain of immunoglobulin (determined by immunodot) (2)
The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.

Avidity :

on rat IgG1: 1 x 109 M-1 - Cf. Avidity sheet, for more details.

Applications :

Cf Reactivity

- can be labelled with biotin, FITC, peroxidase
- can be used as second antibody in immunoassays
- can be used in immunoaffinity chromatography for purification of rat IgG1 (solid phase Sepharose 4B CNBr act.)
- can be used for capture elisa: good binding on plastics
- can be used on cryosections (immunohistology) as first antibody labelled with peroxidase or FITC

Lyophilization :

not tested

References

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunol. 1974,4:44-48

(2) Querinjean P., Bazin H., Starace V., Beckers A., Deckers C. & Heremans J.F. L light chains in rat immunoglobulins. Immunochemistry 1973,10:653-654

Selected References of scientific publications in which MARG1-2 was used: Castedo M. et al. Int. Immunology, 1993, 5: 1569-1576; Soares M. et al. Transplantation 1993, 56: 1427-1433; Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8; Yamada M., et al. Immunology 1993, 78: 298-302; Reding et al. Transplant Immunol. 1994, 2: 231-237; Soares et al., Transplant. Proc. 1995, 27: 282-285.

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