Rat monoclonals against mouse immunoglobulins

Mouse monoclonals against Rat immunoglobulins

Anti Mouse IgG2a gamma2a heavy chain

Mouse IgG1k - clone MARG2a-1

Format	Cat.	Qty
Purified	UPB91540	0.5ml
Purified	UPB91541	1ml
Biotin	UPB91550	0.5ml
Biotin	UPB91551	1ml
FITC	UPB91560	0.5ml
FITC	UPB91561	1ml
Peroxidase	UPB91570	0.5ml
Peroxidase	UPB91571	1ml

Specifications

Immunogen :	IgG2a (Polyclonal antibodies anti horse spleen ferritin, IR33 (1,2)
Avidity :	on rat IgG2a: 2.1 x 109 M-1 - Cf. Avidity sheet, for more details.
Reactivity :	Rat gamma 2a heavy chain of immunoglobulin (determined by immunodot) (2) The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.
Applications :	Cf reactivities - can be used in immunoaffinity chromatography for purification of Rat IgG2a (Solid phase Sepharose 4B CNBr act.) - can be used as second antibody in immunoassays - can be labelled with FITC, biotin, peroxidase - capture in elisa: good binding on plastics - can be coated on nitrocellulose (dot-elispot) - can be used on cryosections (immunohistology) as first antibody labelled with peroxidase or FITC - cannot be frozen and thawed many times. susceptible to be denatured by such a treatment.
Lyophilization :	Yes, with a possible low denaturation.

References

(1) Bazin H., Beckers A., Deckers C. & Moriame M. Transplantable immunoglobulin-secreting tumours in rats.V.Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in the LOU/Wsl rats. J. Nat. Cancer Inst. 1973,51:1359-1361

(2) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology 1974,4:44-48

Selected References of scientific publications in which MARG2a-1 was used: Castedo M. et al. Int. Immunology, 1993, 5: 1569-1576; Soares M. et al. Transplantation 1993, 56: 1427-1433; Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8; Reding et al. Transplant Immunol. 1994, 2: 231-237; Routledge E.G. et al. Transplantation 1995, 60: 847-853; Soares at al., Transplant. Proc. 1995, 27: 282-285.

Mouse IgG1k - clone MARG2a-7

Format	Cat.	Qty
Purified	UPB91580	0.5ml
Purified	UPB91581	1ml
Biotin	UPB91590	0.5ml
Biotin	UPB91591	1ml
FITC	UPB91600	0.5ml
FITC	UPB91601	1ml
Peroxidase	UPB91610	0.5ml
Peroxidase	UPB91611	1ml

Specifications

Immunogen :	IgG2a (Polyclonal antibodies anti horse spleen ferritin, IR33 (1,2)
Avidity :	on rat IgG2a: 6.2 x 109 M-1 (Cf. Avidity sheet, for more details).
Reactivity :	Rat gamma 2a heavy chain of immunoglobulin (determined by immunodot) The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.
Applications :	Cf Reactivity - can be used for capture elisa: good binding on plastics - can be labelled with biotin, peroxidase and FITC - can be used as second antibody in immunoassays - can be coated on nitrocellulose (dot-elispot)
Lyophilization :	Yes, possibly with a low denaturation.

References

(2) BazinH., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology 1974,4:44-48

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