Mouse monoclonals against Rat immunoglobulins

Mouse IgG2ak - clone MARG2c-3

Mouse IgG2ak - clone MARG2c-5

Mouse IgG2ak - clone MARG2c-3

Immunogen :

IgG2c (IR304) (1)

Reactivity :

Rat gamma 2c heavy chain of immunoglobulin (determined by immunodot)
The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.

Avidity :

On IgG2c: 4.8x109 M-1 (Cf. Avidity sheet for more details).

Applications :

Cf Reactivity
- can be used for capture elisa: good binding on plastics
- can be labelled with peroxidase, FITC and biotin
- can be used as second antibody in immunoassays (2,3)
- cannot be frozen and thawed many times. susceptible to be denatured by such a treatment
- cannot be coated on nitrocellulose (dot-elispot)

Lyophilization :

not tested

References

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four sublcasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunol. 1974,4:44-48

(2) Pluschke G. and Bordmann G.. Isolation of rat immunoglobulin class switch variants of rat-mouse hybridomas by enzyme-linked immunosorbent assay and sequential sublining. Eur. J. Immunol. 1987, 17, 413-416

(3) Manouvriez P., Lefebvre M., Genart C., Cormont F. & Bazin H. Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by Elisa with mouse monoclonal antibodies. Immunobiology 1986, 173, 307-308.

Selected Reference : of scientific publication in which MARG2c-3 MAb was used: Castedo M. et al. Int. Immunology 1993, 5: 1569-1576.

Mouse IgG2ak - clone MARG2c-5

Immunogen :

IgG2c (IR304) (1)

Reactivity :

Rat gamma 2c heavy chain of immunoglobulin (determined by immunodot)
The specificity of MAbs anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at an inadequate and unnecessary concentration can induce possible cross-reactions with other subclasses.

Avidity :

On IgG2c: 5.25x109 M-1 (Cf. Avidity sheet for more details).

Applications :

Cf Reactivity
- can be used for capture elisa: good binding on plastics
- can be labelled with biotin and FITC
- can be used as second antibody in immunoassays (2,3)
- can be coated on nitrocellulose (dot-elispot)
- can be used on cryosection (immunohistology) as first antibody (purified)

Lyophilization :

not tested

References

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four sublcasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunol. 1974,4:44-48

(2) Pluschke G. and Bordmann G. Isolation of rat immunoglobulin class switch variants of rat-mouse hybridomas by enzyme-linked immunosorbent assay and sequential sublining. Eur. J. Immunol. 1987, 17, 413-416

(3) Manouvriez P., Lefebvre M., Genart C., Cormont F. & Bazin H. Accurate determination of rat immunoglobulin isotype content in culture supernatants or media by Elisa with mouse monoclonal antibodies. Immunobiology 1986, 173, 307-308.Selected References of scientific publications in which MARG2c-5 MAb was used: Latinne D., et al. Immunol. Rev. 1994, 141: 95-125; Soares et al., Transplant. Proc. 1995, 27: 282-285.

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