Mouse monoclonals against Rat immunoglobulins

Mouse IgG1k - clone MARM-4

Mouse IgG1k - clone MARM-7

Mouse IgG1k - clone MARM-4

Immunogen :

IgM (Polyclonal rat IgM, IR968) (1)

Avidity :

on rat IgM: 3.5 x 109 M-1 (Cf. Avidity sheet, for more details).

Reactivity (2):

Rat mu heavy chain of immunoglobulin (determined by immunodot).
- MARM-4 and MARM-7 do not bind to the same epitope.
- MARM-4 and MARM-7 label the same percentage of IgM rat splenocytes by fluorocytometric analyses.

Cross-reactivity :

MARM-4 doesnít bind to mouse Ig (every isotype) and rabbit IgG.

Applications :

Cf Reactivity

- can be used for capture elisa: good binding on plastics
- can be labelled with peroxidase, biotin, TRITC and FITC
- cannot be used for cell counting using a direct method of immunofluorescence
- can be used as second antibody in immunoassays
- can be used in immunoaffinity chromatography for purification of rat IgM (solid Sepharose 4B CNBr act.)

- can be coated on nitrocellulose (dot-elispot)
- can be used on cryosections (immunohistology) as first antibody labelled with peroxidase or FITC

Lyophilization :

Yes, with a possible low denaturation.

References

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology, 1974,4:44-48.

(2) Cormont F., Manouvriez P., De Clercq L. & Bazin H. The use of rat monoclonal antibodies to characterize, quantify and purify polyclonal or monoclonal mouse IgM. Meth. in Enzymology, 1986,121,622-631.

Selected References of scientific publications in which MARM-4 MAb was used: Platteau B. et al. Int. J. Radiat. Biol., 1989, 55:14; Soares M. et al. Transplantation Proceedings, 1992,24:451-452; Castedo M. et al. Int. Immunol. 1993, 5: 1569-1576; Soares M. et al. Transplantation 1993, 56: 1427-1433; Vanhove B., Bazin H. Immunology 1993, 79: 580-586; Latinne et al. Immunol. Rev. 1994, 141: 95-125; Reding et al. Transplant. Immunol. 1994, 2l: 231-237; Soares et al., Transplantation 1994, 57:1003-1009; Soares et al., Xenotransplantation 1994, 1:118-124; Soares et al. Transplant. Proc. 1994, 26: 1357-1359; Scheringa M. et al., Transplantation 1995, 60: 1350-1353; Soares et al., Transplant. Proc. 1995, 27: 282-285.

Mouse IgG1k - clone MARM-7

Immunogen :

IgM (IR202, IR968) (1)

Reactivity :

Rat mu heavy chain of immunoglobulin (determined by immunodot)
- MARM-7 and MARM-4 do not bind to the same epitope of the rat mu heavy chains.
- MARM-4 and MARM-7 label the same percentage of IgM rat splenocytes by fluorocytometric analyses.

Avidity :

on rat IgM: 1.1 x 109 M-1 . Cf. Avidity sheet, for more details.

Applications :

Cf Reactivity
- can be used for capture elisa: good binding on plastics
- can be used as second antibody in immunoassays
- can be used in immunoaffinity chromatography for purification of rat IgM (Solidphase Sepharose 4B CNBr act.)

Lyophilization :

not tested

References

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology, 1974,4:44-48.

Selected References of scientific publications in which MARM-7 MAb was used: Chavez V. et al. Int. Arch. Allergy Immunol., 1992,97:330-336; Vanhove B., Bazin H. Mol. Immunology, 1992, 29:1-8; Soares M. et al. Transplantation 1993, 56: 1427-1433; Vanhove B., Bazin H. Immunology 1993, 79: 580-586; Soares M. et al. Transplantation 1994, 57: 1003-1009; Latinne D., et al. Immunol. Rev. 1994, 141: 95-125; Soares M. et al. Xeno 1994, 2: 88-92; Soares M., et al. Transplant. Proc. 1994, 26: 1357-1359; Van der Werf W.J. et al. Transplant. Proc. 1994, 26: 1372-1373.

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