Mouse monoclonals against Rat immunoglobulins

Mouse IgG1k - clone MARk-1

Mouse IgG1k - clone MARk-3

Mouse IgG1k - clone LO-Rk1b-1

Mouse IgG1k - clone LO-Rk1b-2

Mouse IgG1k - clone MARk-1

Immunogen :

k L-chain

Reactivity :

Rat k light chain of Ig (determined by immunodot)

Avidity :

on rat k IgD: 1 x 109 M-1 (Cf. Avidity sheet, for more details).

Cross-reactivity :

MARk-1 does not cross react with human IgG coated in ELISA plate. No specific binding on human platelets.

Applications :

Cf Reactivity
- can be used in immunoaffinity chromatography for purification of rat Ig of the k type (Solid phase Sepharose 4B CNBr act.)
- can be used for capture elisa: medium binding on plastics
- can be labelled with peroxidase, biotin, FITC and TRITC
- can be used as second antibody in immunoassays
- cannot be coated on nitrocellulose (dot-elispot)
- can be used on cryosections (immunohistology) as first antibody labelled with peroxidase or FITC

Lyophilization :

Yes.

References

Selected References of scientific publications in which MARk-1 MAb was used: Bazin H. et al. J. Immunol. Methods 1984,66:261-269; Bodeus M. et al. J. Immunol. Methods 1985,79:1-6; khalife J. et al. J. Immunol. 1985,135:2780-2784; Bazin H., Malache J.M. J. Immunol. Meth., 1986,: 19-24; Bazin H. et al. Meth. Enzymol., 1986, 121:638-652; Manouvriez P. et al. Immunobiology 1986, 173, 308-309; Pelletier L. et al. Clin. Exp. Immunol., 1988, 71: 336-342; Castedo M. et al. Int. Immunol. 1993, 5: 1569-1576; Soares M. et al. Transplantation 1993, 56: 1427-1433; Saoudi A. et al. Int. Immunol. 1993, 5: 1559-1567; Vanhove B., Bazin H. Immunology 1993, 79: 580-586; Gmür, R. & Guggenheim, B. J. Dent. Res. 1994, 73: 1421-1428; Reding et al. Transplant Immunol. 1994, 2:231-237; Lepault F. et al. Eur. J. Immunol. 1995, 25:1502-1507; Scheringa M. et al., Transplantation 1995, 60: 1350-1353; Soares M., et al. Transplant. Proc. 1995, 27:282-285.

Mouse IgG1k - clone MARk-3

Reactivity :

Rat k light chain of Ig (allotype Igk-1a) (determined by immunodot)

cross-react weakly in immunofluorescence, elisa and ria with rat k Igk-1b light chain of immunoglobulin.

Avidity :

on rat k 1a IgD: 4.8 x 109 M-1 (Cf. Avidity sheet, for more details).

Applications :

Cf reactivities
- can be used in immunoaffinity chromatography for purification of rat Ig of the k type, Igk-1a allotype (Solid phase Sepharose 4B CNBr act.)
- can be used ofr capture elisa: good binding on plastics

- can be labelled with peroxidase, biotin, alcaline phosphatase, FITC and TRITC
- can be used as second antibody in immunoassays
- cannot be coated on nitrocellulose (dot-elispot)
- can be used on cryosections (immunohistology) as first antibody labelled with FITC (not good when labelled with peroxidase)

Lyophilization :

Yes.

References

(1) Gutman G.A., Bazin H., Rockhlin C.V. & Nezlin R.S. A standard nomenclature for rat immunoglobulin allotypes. Transpl. Proc. 1983,15:1685-1686

Selected References of scientific publications in which MARk-3 MAb was used: Bazin H. et al. J. Immunol. Meth. 1984,71:9-16; Bazin H. et al. J. Immunol. Meth. 1984,66:261-269; Manouvriez P. et al. Immunobiology 1986, 173, 308-309; Bazin H., Malache J.M. J. Immunol. Meth., 1986,: 19-24; Manouvriez P. et al. Mol. Immunol., 1985, 22:1201-1208; Chavez V., et al. Int. Arch. Allergy Immunol., 1992, 97:330-336; Vanhove B., Bazin H. Immunology 1993, 79: 580-586; Fernandes I. et al. Brazilian J. Med. Biol. Res. 1994, 27: 2599-2606; Soares M., et al., Transplant. Proc. 1995, 27: 282-285.

Mouse IgG1k - clone LO-Rk1b-1

Immunogen :

k L-chain of the Igk-1b allotype

Reactivity :

Rat k light chain of immunoglobulin of the Igk-1b allotype (determined by immunodot)

No cross-reaction with rat k light chain on the Igk-1a allotype

Applications :

Cf Reactivity
- can be used for capture elisa: good binding on plastics
- can be labelled with peroxidase
- can be labelled with biotin
- can be labelled with FITC
- can be used as second antibody in immunoassays
- can be used in immunoaffinity chromatography for purification of Rat Igk-1b k Ig (Solid phase Sepharose 4B CNBr act.) (3)

Lyophilization :

not tested

References

(1) Gutman G.A., Bazin H., Rockhlin C.V. & Nezlin R.S. A standard nomenclature for rat immunoglobulin allotypes. Transpl. Proc. 1983,15:1685-1686

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981, Pergamon Press Oxford and N.Y., pp. 615-618

(3) Bazin H., Xhurdebise L.M., Burtonboy G., Lebacq A.M., De Clercq L. & Cormont F. J. Immunol. Rat monoclonal antibodies.I.Rapid purification from in vitro culture supernatants. Meth. 1984,66:261-269

Mouse IgG1k - clone LO-Rk1b-2

Immunogen :

k L-chain of the Igk-1b allotype (1)

Reactivity :

Rat k light chain of immunoglobulin of the Igk-1b allotype (determined by immunodot)
No cross-reaction with rat k light chain of the Igk-1a allotype

Applications :

Cf Reactivity
- can be used in mmunoaffinity chromatography for purification of Rat Igk-1b k Ig (Solid phase Sepharose 4B CNBr act.)(3)
- can be labelled with biotin
- can be used as second antibody in immunoassays
- can be used on cryosections (immunohistology) as first antibody labelled with peroxidase

Lyophilization :

not tested

References

(1) Gutman G.A., Bazin H., Rockhlin C.V. & Nezlin R.S. A standard nomenclature for rat immunoglobulin allotypes. Transpl. Proc. 1983,15:1685-1686

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981, Pergamon Press Oxford and N.Y., pp. 615-618

(3) Bazin H., Xhurdebise L.M., Burtonboy G., Lebacq A.M., De Clercq L. & Cormont F. Rat monoclonal antibodies.I.Rapid purification from in vitro culture supernatants. J. Immunol. Meth. 1984,66:261-269

Selected References of scientific publications in which LO-Rk1b-2 was used: Vanhove B., Bazin H. Immunology 1993, 79: 580-586; Latinne D., et al. Immunol. Rev. 1994, 141: 95-125; Soares et al., Transplant. Proc. 1995, 27:282-285.

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