Mouse monoclonals against Rat immunoglobulins

Mouse IgG1k - clone MARL-15

Immunogen :

Lambda light chain (RH58, IR31) (1,2,3)

Reactivity :

Rat lambda light chain of immunoglobulin (determined by immunodot)

Avidity :

on rat IgG1 l: 2.6 x 1010 M-1 (Cf. Avidity sheet, for more details).

Applications :

Cf Reactivities
- can be used for capture elisa: good binding on plastics
- can be labelled with peroxidase
- can be labelled with FITC and biotin
- can be used as second antibody in immunoassays
- can be used in immunoaffinity chromatography for purification of Rat Ig of the l type
- can be used on cryosections (immunohistology) as first antibody labelled with peroxidase or FITC

Lyophilized :

not tested

References

(1) Bazin H., Beckers A. & Querinjean P. Three classes and four subclasses of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c. Eur. J. Immunology 1974,4:44-48

(2) Querinjean P., Bazin H., Starace V., Beckers A., Deckers C. & Heremans J.F. L light chains in rat immunoglobulins. Immunochemistry 1973,10:653-654

(3) Burtonboy G., Bazin H. & Delferrière N. Rat hybridoma antibodies against canine parvovirus. Archives of Virology 1982,71:291-302

Selected References of scientific publication in which MARL-15 MAb was used: Soares et al., Transplant. Proc. 1995, 27: 282-285

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