FluorIs fluorescence image standard
FluorIs is our array imaging standardisation tool. Whether you want to compare data over a long period of time or exchange data between different labs, FluorIs makes data matchable and reliable.
An
array of almost non bleaching fluorescent spots defined in shape and
intensity is provided for calibration and normalisation purposes. It allows
the direct comparison of array images generated by different fluorescence
reader systems or a single detection system at different time intervals.
Thus detector variances are omitted.
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Due to the characteristic intensity patterns of the FluorIs the sensitivity of the particular system is determined.
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The lateral resolution of the system can be analysed due to the fluorescent resolution test pattern on the surface.
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Calibration of various detection platforms is feasible.
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Array experiments performed at various sites are now comparable.
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Automated data normalisation is performed by application of special IconoClust scripts.
Applied in array image acquisition the principle bears the potential to establish a physical fluorescence standard on the arraying standard. This will enable single dye labelling techniques to overcome existing bottlenecks.
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INTERCHIM DNA Microarray Microarray and gene expression studies |
