SCHOTT Nexterion slides

• Nexterion Slide E

• Nexterion Slide AL

• Nexterion Slide A+

Microarrays are constructed in a way that probe molecules have predefined positions (spots) within the array. Fluorescence labeled target molecules hybridize to the immobilized probe molecules. Subsequently, microarrays are analyzed e.g. by laser-induced fluorescence measurement.

For Nexterion Slides only high quality, laser-cut and extremely low-fluorescent glass substrates are used. The special cleaning and chemical coating procedures favor the generation of high-quality microarrays. The dimensions of all Nexterion Slides are 75.6 mm x 25.0 mm x 1.0 mm.

Nexterion Slides can be used for all spotting systems - contact and contact free printing systems. In combination with our optimized Nexterion Solutions and Buffers, the production of DNA microarrays is a quick and easy task.

Application fields of Nexterion Slides:

• Gene expression monitoring of known and unknown genes

• Mutation detection and analysis

• Genotyping of eukaryotes, microorganisms, viruses etc

• Mapping of genes and clones

 

-	Oligonucleotides				PCR-Products
	Short		Long		-
	with NH2-Link	without NH2-Link	with NH2-Link	without NH2-Link	with NH2-Link	without NH2-Link
Nexterion Slide E	+++	++	+++	+++	++	++
Nexterion Slide AL	++	-	++	-	+++	++
Nexterion Slide A+	+	+	+	++	+	+++
- Not recommended

Advantages

• Homogeneous coating

• Optimal chemistry of functional groups

• Low background fluorescence

• No batch-to-batch variation

• Chemically inert barcode labeling

Homogeneous spotting area is shown for all types of Nexterion Slides.
The dimensions of all Nexterion Slides are:

75.6mm x 25.0mm x 1.0mm

Epoxy Slides are especially suitable for covalent immobilization of amine-modified short oligos (10-25 mers) and non-amine and amine-modified long oligonucleotides (>25 mers). Nexterion Slide E can also be used for printing cDNA PCR-products molecules. Additional amino-modifications of the nucleic acids are not required. The hydrophobic surface allows small spot diameters (100 to 130 µm) to create high density arrays. The surface chemistry is very stable and remains active even during very long spotting runs.

Furthermore, also peptides, proteins (for instance antibodies), cells and tissues can be immobilized on Nexterion Slide E. In principle all nucleophilic groups (NH2-, SH-, OH-) will react immediately and irreversible with the epoxy group. Additional steps for immobilization like UV-crosslinking are not required.

References for Spotting Concentrations The recommended final concentration of nucleic acids in spotting buffers is 10 to 20 uM for obligonucleotides and 0.1 to 0.5 uM for PCR-products. The graph shows the normalized signal intensities after hybridization with different concentrations of labeled oligonucleotides. Oligonucleotides in Nexterion Spot I have been spotted at different concentrations onto Slide E (open squares 5 µM, closed squares 10 µM, open triangles 15 µM, closed triangles 20 µM).

Spot Morphology after hybridization on Nexterion Slide E

Product	Catalog N°	Packaging
Nexterion Slide E, Starter Kit	T50381	1 kit
Inclued : 10 Nexterion Slide E, 10 ml Nexterion Spot, 100 ml Nexterion Block E, 10 ml Nexterion Hyb
Nexterion Slide E, 75.6 mm x 25 mm	U91823	25 slides
Nexterion Slide E, 75.6 mm x 25 mm, barcoded	U91832	25 slides

Nexterion Slide AL are especially suitable for covalent immobilization of PCR products, cDNA molecules, but also for synthetic oligonucleotides. An amino-modification is recommended for efficient immobilization of oligonucleotides. In addition peptides, proteins, antibodies, cells and tissues can be immobilized on Slide AL. The aldehyde group will reversibly react with NH2-groups to form a Schiff's Base. Reduction with sodium borohydride will result in the irreversible immobilization of the nucleic acids and inactivation of un-reacted aldehyde groups.

Nexterion™ Slide A+ is especially suitable for efficient immobilization of PCR products, cDNA molecules and longer, synthetic oligonucleotides (size ≥ 50 mer). The product properties are optimized to support reliable microrarray hybridization results and data analysis by providing high signal intensities and excellent spot morphology.

Nexterion Slide A+ is compatible with most common protocols for aminosilane slides, giving users the opportunity to employ their established microarray processes. It is an ideal substrate, which allows scientists to change PCR product and/or cDNA probes to longer oligonucleotide probes without changing the surface chemistry and protocol. A re-optimization of the entire process for the oligonucleotide applications is not necessary. Thus Nexterion Slide A+ allows an economic transfer not only from PCR products and/or cDNA molecules to longer oligonucleotides, but also from any aminosilane slide to Nexterion Slide A+.

BACK

211 Bis Avenue J.F.Kennedy - BP 1140 - 03103 Montluçon cedex

Tel. (33) (0) 4 70 03 73 06 - interbiotech@interchim.com - http://www.interchim.com