Quality from design to delivery
Whether you need 100, 10,000 or 100,000 custom oligos unmodified, 3', 5' or internally modified and purified, Biosearch can meet your most stringent and exacting synthesis, purification, QA/QC and documentation specifications. Once oligos are designed and sequences are in hand, you can use the checklist below to determine the exact options your particular application requires.
Our oligo design partners can work directly with you to ensure your designed oligos meet stringent and accepted design criteria. These include:
-
Mapping closely to the 3' end
-
Avoidance of sequence repeats and poly A, C, G or T
-
Matched melting temperatures
-
Avoidance of transcript species cross reactivity
Depending on sequence composition, oligos for synthesis can be up to 100 bases long and submitted to Biosearch via email or directly from our website.
Automation, bar coding and robotic synthesis
Upon receipt, 96-well plates of sequences are uploaded electronically to a synthesizer (eliminating human transcription errors) and assigned a unique barcode. Our fully automated robotic synthesizers incorporate advanced control technology and use barcode labels to identify, track, document and archive results of up to 1152 unique custom oligos (12 x 96-well plates of columns) per synthesizer, per run. Automation, bar-coding and the use of the 96-well format from synthesis through delivery guarantees exceptional product quality, confirmation of oligo sequence and unparalleled synthesis to-synthesis consistency.
We are compulsive about quality. Our in-house Chemical Manufacturing Group supplies the majority of reagents used by our DNA Synthesis Group to synthesize your oligos. Both groups generate and maintain extensive lot-to-lot and sequence-related QA/QC records. Every record is generated, retained, archived and retrieved by its unique barcode identification. Biosearch also records and maintains detailed SOPs and test results for all QA procedures including PAGE, HPLC, MALDI-TOF MS and Probe Hybridization options.
Simple RPC clean-up ensures >85% purity
Following synthesis, deprotection and cleavage, your oligos are next routed to our Purification Group. Whether your oligos are moderately purified by RPC (reverse phase cartridge) or stringently purified via HPLC, they are logged, identified, tracked and results archived for docu-mentation using each plate's unique bar-code. By default, every oligo that leaves Biosearch is routinely purified using simple, cost-effective RPC purification to ensure >85% full length oligo purity!
Upon careful successful completion of your order, our Order Processing Group verifies the order I.D., deter-mines required shipping conditions (cold packs, dry ice, etc.), collects all pertinent documentation such as QA/QC, Certificates of Analysis, packing slips, payment invoices and any required customs declarations, and assembles the entire package prior to shipment. We ship world-wide using air express carriers for overnight or second-day air delivery.
|
INTERCHIM DNA Microarray Microarray and gene expression studies |
|
Slides and Reagents for self-printing microarrays
|
