Rat monoclonals against FITC

Anti FITC

 

Rat IgMk - clone LO-FLUO-1

Format

Cat.

Qty

Purified

UPB93140

0.5ml

Purified

UPB93141

1ml

Biotin

UPB93150

0.5ml

Biotin

UPB93151

1ml

FITC

UPB93160

0.5ml

FITC

UPB93161

1ml

Peroxidase

UPB93170

0.5ml

Peroxidase

UPB93171

1ml

 

Specifications

Immunogen :

Mouse k IgG1 labelled with FITC

Reactivity :

Fluorescein isothiocyanate

Avidity :

on FITC labelled BSA: 2 x 1010M-1

Applications :

Cf reactivities
- can be used as second antibody in immunoassays on a first FITC labelled antibody
- can be labelled with biotin and peroxidase
- can be used for capture ELISA (good binding on plastics)
- can be used in in immunofluorescence, can clearly amplify the fluorescence intensity of a direct FITC staining, using the following system: first antibody-FITC + lo-fluo-1-biotin + avidin-FITC

 

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

 

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