Rat monoclonals against Human CD


Anti Human CD1-a (49 kDa) cortical thymocytes

 

Rat IgG2ak - clone LO-CD1-a

Format

Cat.

Qty

Purified

UPB92540

0.5ml

Purified

UPB92541

1ml

Biotin

UPB92550

0.5ml

Biotin

UPB92551

1ml

FITC

UPB92560

0.5ml

FITC

UPB92561

1ml

Peroxidase

UPB92570

0.5ml

Peroxidase

UPB92571

1ml

 

Specifications

Origin :

Hybridoma derived by hybridization of non secreting rat myeloma IR983F (1) cells with spleen cells of a LOU/M rat immunized against human thymocytes.

Isotype :

k IgG2a, allotype Igk-1a.

Antigen Specificity :

CD1-a (PM: 49 kDa)

Epitope :

Does not compete for binding with OkT6 (Ortho-clone).

Cellular Reacitivity (2):

Reacts with most thymocytes.
- Does not react with peripheral blood granulocytes, monocytes, lymphocytes platelets or erythrocytes, tonsil cells.
- Reacts with 25% ALL.
- Does not react with nonT-ALL, AML, B-CLL, B-lymphoma.

Applications :

Identification of T-ALL.

- can be labelled with FITC, biotin

 

References

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618.

(2) Ravoet A.M., Latinne D., Seghers J., Manouvriez P., Ninane J., De Bruyère M., Bazin H., and Sokal G. Methods for analysis of rat monoclonal antibodies directed against human leukocyte differentiation antigens. In "Rat Hybridomas and Rat Monoclonal Antibodies". H. Bazin Ed. CRC Press, Boca Raton, Florida (in press).

(3) Leucocyte Typing IV. White Cell Differentiation Antigens. W. knapp et al. (eds), Oxford University Press, 1989, p. 251, 259, 260, 261, 262, 267.

 

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