Rat monoclonals against Human CD

Rat IgG2ak - clone LO-CD5-a

Origin :

Hybridoma derived by hybridization of non secreting rat myeloma IR983F (1) cells with spleen cells of OkA inbred strain rat immunized against human thymocytes.

Isotype :

k IgG2a, allotype Igk-1b

Epitope :

Inhibits binding of the mouse Leu-1-CD5 MoAb (Becton Dickinson) (2) and of the mouse T1-CD5 MoAb (Coulter).

Cellular Reacitivity (3):

Reacts with all peripheral blood T lymphocytes and a majority of thymocytes.
- Does not react with tonsil B cells, monocytes, granulocytes, platelets or erythrocytes.
- Reacts with T-ALL and weakly with B-CLL, but not with other leukemias.

Applications :

Identification of T-lymphoid cells.
- Quantification of peripheral blood T cells.
- Elimination of T cells in vitro
- can be labelled with FITC, biotin

References

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618.

(2) Engelman et al; (1981) Proc. Natl. Acad; Sci. 78, 1791.

(3) Ravoet A.M., Latinne D., Seghers J., Manouvriez P., Ninane J., De Bruyère M., Bazin H., and Sokal G. Methods for analysis of rat monoclonal antibodies directed against human leukocyte differentiation antigens. In "Rat Hybridomas and Rat Monoclonal Antibodies". H. Bazin Ed. CRC Press, Boca Raton, Florida (in press).

(4) Leucocyte Typing IV. White Cell Differentiation Antigens. W. knapp et al. (eds), Oxford University Press, 1989, p. 331, 332, 335, 336, 338.

Selected Reference : of scientific publication in which LO-CD5-a was used: Alberola-IIa J.,et al. J. Immunol. 1992, 148: 1287-1293.

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