Rat monoclonals against Human CD

Rat IgG2ak - clone LO-CD6-a

Origin :

Hybridoma derived by hybridization of non secreting rat myeloma IR983F (1) cells with spleen cells of a LOU/M rat immunized against the HPB-ALL line.

Isotype :

k IgG2a, allotype Igk-1a

Antigen molecular weight :

129 kDa (HPB-ALL); 105, 125 kDa (T lymphocytes)

Epitope :

LO-CD6-a competes for binding with OkT12 (Ortho Diagnostics)

Cellular Reacitivity (2):

LO-CD6-a reacts with on the average 73% of peripheral blood lymphocytes (92% of T lymphocytes).
- LO-CD6-a does not react with peripheral blood granulocytes, monocytes, platelets or red blood cells.
- LO-CD6-a weakly reacts with T-ALL, B-lymphomas and B-CLL, not with non T-ALL or AML.

Applications :

Identification of mature T cells.
- can be labelled with FITC

References

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618.

(2) Xia H., Ravoet A.M., Latinne D., Ninane J., De Bruyère M., Sokal G., and Bazin H. Rat monoclonal antibodies specific for human T lymphocytes. In "Rat Hybridomas and Rat Monoclonal Antibodies". H. Bazin Ed. CRC Press, Boca Raton, Florida (in press).

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