Rat monoclonals against Human CD

Rat IgG2bk - clone LO-panB-a(CDw78)

Origin :

Hybridoma derived by hybridization of non secreting rat myeloma IR983F (2,3) cells with spleen cells of a LOU/M rat (1) immunized against human B chronic lymphocytic leukaemia cells.

Cellular Reacitivity (2):

Reacts with B lymphocytes from peripheral blood, tonsil, spleen, and activated B cells (EBV, SAC)
- Does not react with T cells, monocytes, granulocytes, platelets or erythrocytes.
- Reacts with B-CLL, Burkittís lymphoma, most non-T ALL and PHA-PBL.

Applications :

- Identification of B malignancies.
- Quantification of B cells.
- In vitro elimination of B cells.
- can be labelled with FITC
- can be used on cryosections (immunohistology) as first antibody labelled with FITC (5)

References

(1) Bazin H., Deckers C., Beckers A., Heremans J.F. Transplantable immunoglobulin-secreting tumours in rats.I.General features in LOU/Wsl Strain rat immunocytomas and their monoclonal proteins. Int. J. Cancer, 1972, 10: 568-580.

(2) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618.

(3) Lebacq-Verheyden A.M., Neirynck A., Ravoet A.M., Bazin H. Rat hybridoma technology: culturing of rat myeloma cell line IR983F prior to cell fusion. Hybridoma, 1983, 2: 355-358.

(4) Leucocyte Typing IV. White Cell Differentiation Antigens. W. knapp et al. (eds), Oxford University Press, 1989, p. 122,126,142,154,165,200,219,224.

(5) Bombil et al. Cancer Immunol. Immunother., 1995, 40: 383-389.

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