Rat monoclonals against Human HLA Class II

Rat IgG2bk - clone LO-DRa

Origin :

Hybridoma derived by hybridization of non secreting rat myeloma IR983F (1) cells with spleen cells of a LOU/M rat immunized against human peripheral lymphocytes activated by phytohemagglutinin.

Antigen Specificity :

HLA-class II ; p29: 34kDa

Epitope :

Inhibits binding of the mouse OkIa MoAb (Ortho-clone). (2)

Cellular Reactivity :

Reacts with B lymphocytes of peripheral blood, tonsils and spleen.
- Reacts with dendritic cells, monocytes and activated T lymphocytes.
- Does not react with peripheral T lymphocytes, granulocytes, platelets or erythrocytes.
- Reacts with non-T leukemias and non-T lymphomas and with most AML.

Applications :

Detection of leukaemic non-T lymphoblast and myeloblasts. Quantification of B cells.
- can be labelled with FITC, biotin
- can be used on cryosections (immunohistology) as first antibody (purified, revealed by MARG2b-3 PO)
- can be used on paraffin sections (immunoperoxidase)

References

(1) Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618.

(2) Reinherz et al. (1979) J. Exp. Med. 150, 1472.

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