Rat monoclonals against Human immunoglobulins

Anti Human IgA alpha1 heavy chain

 

Rat IgG1k - clone LO-HA-8

Rat IgG2ak - clone LO-HA-9

 

Rat IgG1k - clone LO-HA-8

Format

Cat.

Qty

Purified

UPB92100

0.5ml

Purified

UPB92101

1ml

Biotin

UPB92110

0.5ml

Biotin

UPB92111

1ml

FITC

UPB92120

0.5ml

FITC

UPB92121

1ml

Peroxidase

UPB92130

0.5ml

Peroxidase

UPB92131

1ml

 

Specifications

Immunogen :

Purified Human IgA

Reactivity :

Human alpha 1 heavy chain of immunoglobulin.

Avidity :

on human IgA l BRAI monoclonal myeloma protein: 1.9 x 109 M-1

Cross-reactivity :

Does not cross-react with guinea pig, chicken, rabbit, goat, sheep, pig, cow, horse, dog, swine Ig (ELISA test).

Applications :

Cf Reactivity
- can be used for capture elisa: good binding on plastics
- can be labelled with biotin, FITC and peroxidase
- can be used as second antibody in immunoassays
- can detect membrane IgA on human b lymphocytes

 

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

 

Rat IgG2ak - clone LO-HA-9

Format

Cat.

Qty

Purified

UPB92140

0.5ml

Purified

UPB92141

1ml

Biotin

UPB92150

0.5ml

Biotin

UPB92151

1ml

FITC

UPB92160

0.5ml

FITC

UPB92161

1ml

Peroxidase

UPB92170

0.5ml

Peroxidase

UPB92171

1ml

 

Specifications

Immunogen :

Purified Human IgA

Reactivity :

Human alpha 1 and alpha 2 heavy chain of immunoglobulin.

Cross-reactivity :

Does not cross-react with guinea pig, chicken, rabitt, goat, sheep, pig, cow, horse, dog, swine Ig. Does not cross-react with mouse Ig (ELISA test).

Avidity :

on human IgA l BRAI monoclonal myeloma protein: 5 x 108M-1

Applications :

Cf Reactivities
- can be used for capture elisa: good binding on plastics
- can be labelled with biotin
- can be used as second antibody in immunoassays
- can detect membrane IgA on human lymphocytes
- can be used in immunoaffinity chromatography for purification of human IgA (Solid phase Sepharose 4B CNBr act.)

 

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

 

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