Rat monoclonals against Human immunoglobulins


Anti Human IgM mu heavy chain

 

Rat IgMk - clone LO-HM-7

Rat IgMk - clone LO-HM-14

 

Rat IgMk - clone LO-HM-7

Format

Cat.

Qty

Purified

UPB91900

0.5ml

Purified

UPB91901

1ml

Biotin

UPB91910

0.5ml

Biotin

UPB91911

1ml

FITC

UPB91920

0.5ml

FITC

UPB91921

1ml

Peroxidase

UPB91930

0.5ml

Peroxidase

UPB91931

1ml

 

Specifications

Immunogen :

Purified Human IgM

Reactivity :

Human mu heavy chain of immunoglobulin

Avidity :

on human IgM monoclonal myeloma protein: 2.9 x 109M-1

Cross-reactivity :

Does not cross-react with mouse Ig and chicken, rabbit, goat, sheep, bovine, horse, dog IgG. Does not cross-react with baboon Ig (ELISA test).

Applications :

Cf Reactivity- can be used for capture elisa (good binding on plastics)
- can be labelled with FITC, biotin and peroxidase
- can be used as second antibody in immunoassays
- can detect membrane IgM on human b lymphocytes

- can be used in immunoaffinity chromatography for purification of Human IgM (Solid phase Sepharose 4B CNBr act.)
- can be used on cryosections (immunohistology) as first antibody labelled with peroxidase

 

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618;

Selected References of scientific publications in which LO-hM-7 was used: Vanhove B., Bach F.H. Transplantation 1993, 56: 1251-1292; Latinne D. et al. Immunol. Rev. 1994, 141: 95-125


 

Rat IgMk - clone LO-HM-14

Format

Cat.

Qty

Purified

UPB91940

0.5ml

Purified

UPB91941

1ml

Biotin

UPB91950

0.5ml

Biotin

UPB91951

1ml

FITC

UPB91960

0.5ml

FITC

UPB91961

1ml

Peroxidase

UPB91970

0.5ml

Peroxidase

UPB91971

1ml

 

Specifications

Immunogen :

Purified polyclonal Human IgM

Reactivity :

Human mu heavy chain of immunoglobulin.

Avidity :

on human IgM monoclonal myeloma protein: 2.4 x 109M-1

Applications :

Cf Reactivity
- can be used for capture elisa: good binding on plastics
- can be labelled with biotin and peroxidase
- can be used as second antibody in immunoassays

 

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

Selected Reference : of scientific publication in which LO-hM-7 was used: Vanhove B., Bach F.H. Transplantation 1993, 56: 1251-1292

 

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