Rat monoclonals against Mouse immunoglobulins

Anti Mouse IgA alpha heavy chain

 

Rat IgMk - clone LO-MA-7

Rat IgMk - clone LO-MA-10

 

Rat IgMk - clone LO-MA-7

Format

Cat.

Qty

Purified

UPB90250

0.5ml

Purified

UPB90251

1ml

Biotin

UPB90260

0.5ml

Biotin

UPB90261

1ml

FITC

UPB90270

0.5ml

FITC

UPB90271

1ml

Peroxidase

UPB90280

0.5ml

Peroxidase

UPB90281

1ml

 

Specifications

Immunogen :

purified polyclonal IgA (Mouse IgA) from BALB/c mice

Reactivity :

Mouse alpha heavy chain of immunoglobulin (determined by immunodot).

Cross-reactivity :

does not bind to human IgG and IgM. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG (ELISA test).

Avidity :

on mouse IgA: 4.8 x 109 M-1

Applications :

Cf Reactivity
- can be labelled with Biotin and Peroxidase
- cannot be labelled with FITC
- can be used as second antibody in immunoassays
- capture Elisa: good binding on plastics
- can be used in mmunoaffinity chromatography for purification of mouse IgA (solid phase Sepharose 4b CNBr act.) - capacity: see data sheet.

Lyophilization :

not tested

 

References

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

 

Rat IgMk - clone LO-MA-10

Format

Cat.

Qty

Purified

UPB90290

0.5ml

Purified

UPB90291

1ml

Biotin

UPB90300

0.5ml

Biotin

UPB90301

1ml

FITC

UPB90310

0.5ml

FITC

UPB90311

1ml

Peroxidase

UPB90320

0.5ml

 

Specifications

Immunogen :

purified polyclonal IgA from BALB/c mice

Reactivity :

Mouse alpha heavy chain of immunoglobulin (determined by immunodot), positively tested on BALB/c and C57B1/7 mouse sera

Cross-reactivity :

does not bind to human IgG and IgM. Does not bind to chicken, rabbit, goat, sheep, bovine, horse, dog, swine, baboon IgG (ELISA test).

Avidity :

on mouse IgA: 2.9 x 109M-1

Applications :

Cf Reactivity
- capture Elisa: good binding on plastics
- can be labelled with Peroxidase and biotin
- can be labelled with FITC
- can be used as second antibody in immunoassays
- can be coated on nitrocellulose (dot-elispot)
- can be used on cryosections (immunohistology) as first antibody labelled with peroxidase

Lyophilization :

not tested

 

References 

Bazin H. Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line. Prot. Biol. Fluids, 1982, Peeters E. ed., 29th colloquium 1981 Pergamon Press Oxford and N.Y.: 615-618

 

BACK

TOP

211 bis, avenue J.F. Kennedy - BP 1140 - 03103 Montluçon cedex - France

Hot line (33) (0) 4 70 03 73 06  - Tel. (33) (0) 4 70 03 88 55 - Fax. (33) (0) 4 70 03 82 60

interbiotech@interchim.com - http://www.interchim.com